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在猪绦虫融合抗原 pCC2 7(本室从六钩蚴cDNA表达文库筛选的 3个保护性抗原经拼接所得 )基因片段 5′末端引入 1个通用型辅助性T淋巴细胞表位、2个谷胱甘肽还原酶的辅助性T淋巴细胞表位 (TGG) ,构建成DNA疫苗。通过肌肉注射途径将这种DNA疫苗免疫小鼠和仔猪。动物试验结果表明 ,TGG表位可提高小鼠血清 pCC2 7抗体IgG、IgG1、IgG2a的水平 ,且长时间保持较高应答水平。用该疫苗免疫仔猪获得了 89%的保护率。
A Universal T-helper T cell epitope was introduced into the 5 ’end of the gene fragment of Taenia solium fusion antigen pCC2 7 (three protective antigens screened from the cDNA library of Alhuria toxoplasmosis) Glycopeptide reductase helper T lymphocyte epitope (TGG), constructed into a DNA vaccine. The DNA vaccine was used to immunize mice and piglets by intramuscular injection. The results of animal experiments show that TGG epitopes can raise the levels of IgG, IgG1 and IgG2a in mouse serum of pCC2 7, and maintain a high response level for a long time. Piglets immunized with this vaccine achieved 89% protection.