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目的:探讨加味麻黄定喘汤对哮喘小鼠气道炎症和Thl/Th2类细胞因子变化的影响及其可能机制。方法:雄性BALB/c小鼠32只随机分成4组,即正常组、模型组和加味麻黄定喘汤低、高剂量组。分别采用酶联免疫吸附法(ELISA)和蛋白质印迹检测支气管肺泡灌洗液(BALF)中白细胞介素-4(IL-4)、IL-5、IL-13及γ-干扰素(IFN-γ)含量,并观察BALF中炎症细胞和肺组织病理学改变。免疫组化法检测细胞外信号调节蛋白激酶(ERK)在肺内表达。结果:模型组小鼠BALF中炎症细胞计数、IL-4、IL-5、IL-13水平升高,IFN-γ水平降低,与正常组比较,差异有统计学意义(P<0.05)。加味麻黄定喘汤低、高剂量组小鼠BALF中炎症细胞计数、IL-4、IL-5、IL-13水平降低,IFN-γ水平明显上升,与模型组比较,差异有统计学意义(P<0.05)。加味麻黄定喘汤能明显减轻哮喘小鼠肺组织病理学改变,加味麻黄定喘汤低、高剂量组之间比较,有显著性差异(P<0.05)。模型组ERK在肺内表达较正常组增强,加味麻黄定喘汤低、高剂量组肺组织中ERK的蛋白质印迹表达均较模型组明显下降(P<0.05)。结论:加味麻黄定喘汤对哮喘小鼠具有保护作用,其机制可能与抑制ERK磷酸化,调节IL-4/IFN-γ平衡失调、减轻炎症细胞浸润有关。
Objective: To investigate the effects of Modified Mahuang Dingchuan Tang on the changes of airway inflammation and Th1 / Th2 cytokines in asthmatic mice and its possible mechanism. Methods: Thirty-two male BALB / c mice were randomly divided into 4 groups: normal group, model group and Modified Mahuang Dingchu Tang low and high dose groups. The levels of interleukin-4, IL-5, IL-13 and IFN-γ in bronchoalveolar lavage fluid (BALF) were detected by ELISA and Western blot, ) Content, and observed in BALF inflammatory cells and lung tissue pathological changes. Immunohistochemistry was used to detect the expression of extracellular signal-regulated protein kinase (ERK) in the lung. Results: The number of inflammatory cells, the levels of IL-4, IL-5 and IL-13 in BALF and the levels of IFN-γ in BALF were lower in model group than those in normal group (P <0.05). The levels of inflammatory cytokines, IL-4, IL-5 and IL-13 in BALF of low and high dosages of Modified Mahuang Dingchu Tang were decreased and the level of IFN-γ was significantly increased in BALF of mice. Compared with model group, the difference was statistically significant P <0.05). Modified Mahuang Dingchuan Decoction can significantly reduce the pathological changes of lung tissue in asthmatic mice, add flavored Mahuang Dingchuang low and high dose group, there was a significant difference (P <0.05). The expression of ERK in the model group was higher than that in the normal group. The expression of ERK in the lungs of the low and high dose flavonoids group was significantly lower than that of the model group (P <0.05). Conclusion: Modified Mahuang Dingchuantang protective effect on asthmatic mice may be related to inhibition of phosphorylation of ERK, regulation of imbalance of IL-4 / IFN-γ and alleviation of inflammatory cell infiltration.