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骨骼肌是鱼体的主要组成部分,也是衡量鱼体生长发育的重要指标。为了解鳜(Siniperca chuatsi)早期发育阶段骨骼肌纤维的生长发育特征,通过制作孵化后1～41日龄个体骨骼肌背右侧第一肌节石蜡切片,利用图像分析软件统计该肌节中肌纤维的数目和面积,分析了鳜骨骼肌纤维的增生和肥大生长特征。结果表明,鳜早期发育阶段骨骼肌生长同时包括数目增加(增生)和面积增大(肥大),背侧第一肌节中肌纤维总数由40个增加到520个,肌纤维总面积由805.30μm2增加到186 422.77μm2。其中,孵化后1～9日龄,肌纤维相对增生数目下降,相对增加面积缓慢上升,相对增加面积中增生生长的贡献率由60.53%降至8.80%,肥大生长则与之相反;9～15日龄,肌纤维相对增生数目呈现上升,相对增加面积相对平缓,相对增加面积中增生生长的贡献率略呈上升(由8.80%上升至20.26%),肥大生长贡献率略有下降;15～41日龄,相对增生数目和相对增加面积均呈上升趋势,相对增加面积中增生生长的贡献率下降并趋于平稳,肥大生长贡献率由79.79%上升到87.41%,成为优势生长。
Skeletal muscle is the main part of the fish, but also an important indicator of the growth and development of fish. In order to understand the growth and development of skeletal muscle fibers during the early developmental stage of Siniperca chuatsi, the first sarcomere paraffin section of the right dorsal muscle of skeletal muscle of individuals aged 1 to 41 days after hatching was prepared, and the image analysis software was used to calculate the skeletal muscle fibers Number and area, analysis of skeletal muscle fiber hyperplasia and hypertrophy growth characteristics. The results showed that in the early stage of development, the skeletal muscle growth at the same time increased both in number and hyperplasia (hypertrophy). The total number of myofibers in the first dorsal part increased from 40 to 520 and the total area of myofibers increased from 805.30μm2 186 422.77 μm2. Among them, 1 to 9 days after hatching, the relative number of myofibroblasts decreased, the relative increase of the area increased slowly, the relative increase in the area of the proportion of the contribution of the growth rate from 80.53% to 8.80%, while the opposite growth; The relative proliferation area increased relatively (from 8.80% to 20.26%), and the contribution rate of hypertrophy decreased slightly. The contribution rate of growth of 15 to 41 days , The number of relative proliferation and the relative increase area all showed an upward trend. The contribution rate of the growth in the relative increase area decreased and stabilized, and the contribution rate of growth of hypertrophy rose from 79.79% to 87.41%, which became the dominant growth.