论文部分内容阅读
家蚕细胞质多角体病毒(Cytoplasmic polyhedrosis virus以下简称CPV)颗粒中含有以双链RNA为模板的RNA多聚酶,于体外合成系统中可以获得毫克量的CPV单链RNA.体外合成反应液经DEAE-Sephadex A-25柱层析分离、纯化、浓缩,再经2.5％聚丙烯酰胺凝胶电泳分离,表明体外合成的单链RNA含有九条区带.体外合成的单链RNA,于小麦胚无细胞制剂翻译系统中给予同位素标记的氨基酸,翻译的蛋白与家蚕CPV抗血清产生免疫沉淀线,说明体外系统转录的单链RNA含有足够的基因信息,指导家蚕CPV的结构蛋白的合成.
Cytoplasmic polyhedrosis virus (CPV) particles contain double-stranded RNA as a template RNA polymerase in vitro synthesis system can be obtained in milligram of CPV single-stranded RNA in vitro synthesis of the reaction solution by DEAE-Sephadex A -25 column chromatography, purified, concentrated, and then separated by 2.5% polyacrylamide gel electrophoresis, indicating that in vitro synthesis of single-stranded RNA contains nine bands in vitro synthesis of single-stranded RNA in wheat germ cell-free preparation of translation system The purified protein and silkworm CPV antiserum produced immunoprecipitation lines, which indicated that the single-stranded RNA transcribed in vitro contained sufficient gene information to guide the synthesis of the structural protein of silkworm CPV.