使用这个标记系统方法简单,迅速见效率高。将DNA变性后仅需10分钟就可完成最佳标记反应。将新制备的(BACH)水溶液(10mg/ml)12.5μl和5%GA水溶液15μl加到25μl变性噬菌体DNA液(1mg/ml)中。混合液37℃温育10分钟,反应完成后按常规沉淀法纯化DNA,贮存于-20℃。 This method of marking the system is simple and fast and efficient. The optimal labeling reaction can be completed in 10 minutes by denaturing DNA. 12.5 μl of freshly prepared (BACH) aqueous solution (10 mg / ml) and 15 μl of 5% GA aqueous solution were added to 25 μl of denatured phage DNA solution (1 mg / ml). The mixture was incubated at 37 ° C for 10 minutes. After completion of the reaction, the DNA was purified by conventional precipitation and stored at -20 ° C.