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目的了解开封市肉猪养殖屠宰加工过程中沙门菌的污染状况。方法采集不同来源的养殖场猪混合粪便、成年猪肛拭子、胴体涂抹物、肠系膜淋巴结样本,共187份,按照GB 4789.4—2010进行沙门菌的分离和鉴定。结果猪混合粪便沙门菌检出率为4.5%,肛拭子沙门菌的检出率为16.4%,胴体涂抹物沙门菌检出率为34.5%,肠系膜淋巴结沙门菌检出率为27.3%。不同季节的检出率差异有统计学意义(P<0.05)。阳性分离株分布于沙门菌的5个群,共14个血清型,以阿贡纳沙门菌和德尔卑沙门菌为主。结论屠宰环节是沙门菌交叉污染的关键点,应采取综合措施,有效控制猪肉沙门菌污染。
Objective To understand the status of Salmonella contamination in slaughter and processing of hoggery in Kaifeng City. Methods A total of 187 samples of swine mixed feces, adult swine swabs, carcass smears and mesenteric lymph nodes were collected from farms of different origins. A total of 187 samples were obtained, and Salmonella was isolated and identified according to GB 4789.4-2010. Results The detection rate of Salmonella mixed with swine in swine was 4.5%, the detection rate of Salmonella spp. Was 16.4%, the detection rate of Salmonella in carcass was 34.5%, and the detection rate of Salmonella in mesenteric lymph node was 27.3%. The detection rate of different seasons was significantly different (P <0.05). The positive isolates were distributed in 5 groups of Salmonella, a total of 14 serogroups, with Salmonella gonorrhoeae and Salmonella delphi dominated. Conclusion Slaughtering is the key point of cross-contamination of Salmonella, and comprehensive measures should be taken to control the contamination of Salmonella in pork.