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目的:建立高效液相色谱法同时测定毛冬青药材中2个三萜皂苷类成分(毛冬青皂苷B1和毛冬青皂苷B2)的含量。方法:采用Phenomenex C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-0.2%磷酸水溶液为流动相梯度洗脱,流速1.0 mL.min-1,检测波长203 nm。结果:毛冬青皂苷B1和毛冬青皂苷B2线性范围分别为2.57～12.82μg(r=0.9998)和4.51～22.52μg(r=0.9999),平均回收率(n=6)分别为100.6%(RSD=0.94%)和100.7%(RSD=1.5%)。结论:6批样品的测定结果表明,该方法简便、准确,可用于毛冬青药材中毛冬青皂苷B1和毛冬青皂苷B2的含量测定。
Objective: To establish a HPLC method for the simultaneous determination of two triterpene saponins (Mao hollysin B1 and Mao hollysin B2) in Mao Dongqing medicinal materials. Method: The mobile phase was eluted with a mobile phase of acetonitrile-0.2% phosphoric acid with a flow rate of 1.0 mL · min-1 on a Phenomenex C18 column (4.6 mm × 150 mm, 5 μm) with a detection wavelength of 203 nm. RESULTS: The linear ranges of Mao hollysin B1 and Mao hollysin B2 were 2.57 ~ 12.82μg (r = 0.9998) and 4.51 ~ 22.52μg (r = 0.9999), respectively. The average recoveries (n = 6) were 100.6% 0.94%) and 100.7% (RSD = 1.5%). Conclusion: The determination results of six batches of samples show that the method is simple and accurate and can be used for the determination of Mao hollysin B1 and Mao hollysin B2 in Mao Dong Qing.