利用组织培养方法研究神经组织的再生和分化、生长和发育等问题有其优越性,因能更好地控制神经元的环境,以利于研究促进或抑制神经细胞分裂和分化的因素和条件。我们选用颈上节作为培养材料,是考虑它属于植物性神经系统外周部分,其化学组织结构比较简单,颈上节又比其他交感节大,容易着手;而且自从Levi-Montalcini等(1954)发现一种能刺激交感神经元(及感觉神经元)生存和生长发育的特殊旦白质称神经生长因子(nerve growth factor,简称NGF)之后,人们可利用NGF作为培养 The use of tissue culture methods to study the regeneration and differentiation, growth and development of neural tissue has its advantages because it can better control the neuronal environment and facilitate the study of the factors and conditions that promote or inhibit neural cell division and differentiation. We choose the cervical section as a culture material, consider it belongs to the peripheral part of the autonomic nervous system, its chemical structure is relatively simple, the neck section than other sympathetic large, easy to handle; and since Levi-Montalcini (1954) found A special denatured nerve growth factor (NGF), which stimulates the survival and growth of sympathetic and / or sensory neurons, can be used to culture NGF