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鲎试验(简称LLT)是近十几年发展起来的检测微量内毒素的一种方法。1956年Bang发现给美洲鲎注入革兰氏阴性杆菌,引起鲎血凝固。1964年Bang与Levin等进一步证实,革兰氏阴性杆菌的微量内毒素可以使鲎变形细胞溶解物产生凝胶。LeVin等用氯仿提取革兰氏阴性杆菌败血症病人血浆中的内毒素,进行此试验,用于临床检测病人的内毒素血症。近年来通过一些临床检验,实践证明这一非特异性试验简单、灵敏,有助于革兰氏阴性杆菌感染的快速诊断。但Butler等用LLT检测伤寒病人血浆中的内毒素时,发现全组21名未经抗菌治疗的病人均呈现阴性反应。该氏认为伤寒患者的持续发热和毒血症症状,主要不是由内毒素血症所引起。然而伤寒
鲎 test (referred to as LLT) is developed more than a decade of trace amounts of endotoxin detection method. In 1956, Bang discovered that Gram-negative bacilli were injected into the American pomfret, causing blood coagulation. In 1964, Bang and Levin further confirmed that the micro-endotoxin of Gram-negative bacilli can produce gelatinous gel in the cell lysate. LeVin et al. Extracted endotoxin in the plasma of patients with Gram-negative bacilli septicemia using chloroform and performed this test for the clinical detection of endotoxemia in patients. In recent years through a number of clinical tests, the practice proved that this non-specific test is simple and sensitive, contribute to the rapid diagnosis of Gram-negative bacilli. However, Butler and other LLT test for typhoid plasma endotoxin patients, found that the entire group of 21 patients without antibacterial therapy were negative. The think that typhoid fever patients and persistent symptoms of toxemia, mainly not caused by endotoxemia. However, typhoid fever