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本实验室应用培养大鼠黄体细胞的方法,研究酪氨酸及其衍生物对hCG 致孕酮生成作用的影响,以分析酪氨酸的作用是否通过其衍生物而发挥的。用PMSG(65U/鼠)和hCG(35U/鼠)处理25～28天雌性大鼠造成超排卵并形成黄体,取hCG 处理后7天的黄体制备悬液,加入各种试剂共同孵育1.5小时后用放免法测定孕酮含量。结果表明,酪氨酸对大鼠离体黄体细胞具有明显的抗hCG 致孕酮生成作用,而酪氨酸的衍生物,多巴胺和去甲肾上腺素,3-碘-酪氨酸和3,5-二溴-酪氨酸则与酪氨酸作用相反,这说明,酪氨酸对黄体细胞孕酮生成的抑制作用是其本身的作用,而不是通过其衍生物而产生的。
In our laboratory, the method of culturing rat luteal cells was used to study the effect of tyrosine and its derivatives on the production of progesterone induced by hCG to analyze whether tyrosine functions through its derivatives. Female rats treated with PMSG (65U / mouse) and hCG (35U / mouse) for 25-28 days resulted in superovulation and corpus luteum formation. The luteal preparation suspension was prepared 7 days after hCG treatment and incubated with various reagents for 1.5 hours Determination of progesterone by radioimmunoassay. The results showed that tyrosine had obvious anti-hCG-induced progesterone production in isolated rat luteal cells, while tyrosine derivatives, dopamine and norepinephrine, 3-iodo-tyrosine and 3,5 - dibromo-tyrosine and tyrosine on the contrary, indicating that tyrosine on progesterone progesterone production inhibition is its own role, rather than through its derivatives.