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目的 探讨 1,2 5 -二羟基维生素 D3 对环磷酰胺促发的 NOD鼠 1型糖尿病的预防作用。方法 N OD鼠从离乳后隔日接受 1,2 5 -二羟基维生素 D3 治疗 ,第 10周龄给予环磷酰胺 30 0 m g/ kg,观察 1,2 5 -二羟基维生素 D3 对环磷酰胺处理的 NOD鼠糖尿病发病率和胰岛炎的影响 ,及对 Th1、Th2细胞因子 m RNA表达的影响。结果 1,2 5 -二羟基维生素 D3 处理组糖尿病发病率为 17% ,明显低于对照组 6 7% (P<0 .0 5 ) ,且胰岛炎严重程度也明显减轻。处理组胰腺 TNT- α、IFN- γ m RNA的表达较对照组明显降低 ,而 IL- 10 m RNA的表达无明显改变。结论 1,2 5 -二羟基维生素 D3 可以预防 NOD鼠环磷酰胺诱发的糖尿病的发生 ,其机制可能与纠正 Th1型细胞因子与 Th2型细胞因子比例失衡有关
Objective To investigate the preventive effects of 1,2 5 -dihydroxyvitamin D3 on cyclophosphamide-induced type 1 diabetes in NOD mice. Methods N 2 O rats were treated with 1,2 5 - dihydroxyvitamin D3 every other day after weaning and 30 0 mg / kg cyclophosphamide at 10 weeks old. The effects of 1,2 5 - dihydroxyvitamin D3 on cyclophosphamide The incidence of diabetic and insulitis in NOD mice and the effect on the expression of Th1 and Th2 cytokines. Results The incidence of diabetes in 1,25 - dihydroxyvitamin D3 group was 17%, significantly lower than that in the control group (67%, P <0.05), and the severity of insulitis was also significantly reduced. The expression of TNT-α and IFN-γmRNA in the pancreas of the treated group was significantly lower than that of the control group, while the expression of IL-10 mRNA did not change significantly. Conclusion 1,25 - dihydroxyvitamin D3 can prevent cyclophosphamide - induced diabetes mellitus in NOD rats, and its mechanism may be related to the imbalance of Th1 cytokines and Th2 cytokines