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应用分子克隆技术,构建重组腺病毒质粒Adtrack-canstatin(Ad-can),并建立人卵巢上皮性癌移植瘤动物模型.动物模型建立后,将荷瘤裸鼠随机分为3组,Ad-Can、Ad-GFP和PBS对照组,每组6只.治疗采用肿瘤内局部多点注射4.0×1010PFU/ml病毒上清液或PBS液0.1 ml,共2次,注射间隔72小时.8天后肿瘤体积出现明显差异,Ad-can组明显小于对照组,P<0.01,而Ad-GFP和PBS对照组间无明显差异,P>0.05.另外,Ad-can组CD105明显减少,微血管密度降低,P<0.05.结果提示:Canstatin基因能明显抑制人卵巢上皮性癌的生长,其机制可能是通过抑制肿瘤新生血管形成起作用的.
The recombinant adenovirus plasmid Adtrack-canstatin (Ad-can) was constructed and the animal model of human ovarian epithelial carcinoma xenografts was established by molecular cloning technique.After establishment of the animal model, nude mice bearing tumor were randomly divided into three groups: Ad-Can , Ad-GFP and PBS control group, with 6 in each group.The treatment was intradermal injection of 4.0 × 1010 PFU / ml viral supernatant or 0.1 ml PBS solution for 2 times at 72 hours after injection.The tumor volume Ad-can group was significantly smaller than the control group, P <0.01, but no significant difference between Ad-GFP and PBS control group, P> 0.05. In addition, Ad-can group CD105 was significantly reduced, microvessel density decreased, P < 0.05.The results suggest that Canstatin gene can significantly inhibit the growth of human epithelial ovarian cancer and its mechanism may be through the inhibition of tumor angiogenesis.