论文部分内容阅读
甲羟戊酸(MVA)途径是萜类化合物生物合成的重要途径,该研究在厚朴转录组测序基础上,利用生物信息学方法对MVA途径中相关基因Mo ACOT,Mo HMGS,Mo HMGR,Mo MK进行全面的生物信息学分析。结果表明,Mo ACOT和Mo MK为稳定的亲水蛋白,而Mo HMGS,Mo HMGR为不稳定的亲水蛋白;二级结构均为混合型结构的蛋白质,α-螺旋是所有基因多肽链中大量的结构元件;利用同源建模法对三级空间结构进行了预测;跨膜域分析显示Mo ACOT,Mo HMGS,Mo MK无跨膜区,MoHMGR具有2个跨膜区,分别为39~61 aa和82~104 aa;系统进化树分析表明Mo ACOT,Mo HMGS,Mo HMGR,Mo MK均与被子植物或双子叶植物遗传距离较近,符合遗传进化规律;基因表达水平显示Mo ACOT,Mo HMGS,Mo HMGR,Mo MK表达水平在厚朴与凹叶厚朴间均无显著差异。研究结果为解析厚朴萜类生物合成MVA途径的分子机制提供理论依据。
The mevalonate (MVA) pathway is an important pathway for the biosynthesis of terpenoids. Based on the Magnolia officinalis transcriptome sequencing, bioinformatics methods were used to analyze the molecular mechanisms of MVA pathway such as Mo ACOT, Mo HMGS, Mo HMGR, Mo MK conducted a comprehensive bioinformatics analysis. The results showed that Mo ACOT and Mo MK were stable hydrophilic proteins, whereas Mo HMGS and Mo HMGR were unstable hydrophilic proteins. The secondary structures were both of mixed-type proteins. The α-helix was a large amount of all polypeptide chains The results of transmembrane domain analysis showed that Mo ACM, Mo HMGS and Mo MK had no transmembrane domain, and MoHMGR had two transmembrane domains, ranging from 39 to 61 phylogenetic tree analysis showed that the genetic distance between Mo ACOT, Mo HMGS, Mo HMGR and Mo MK was closer to that of angiosperms or dicotyledons, and the gene expression level showed Mo ACOT, Mo HMGS , The expression levels of Mo HMGR and Mo MK were not significantly different between Magnolia officinalis and Magnolia officinalis. The results provide a theoretical basis for the molecular mechanism of the MVA pathways of terpene biosynthesis.