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研究LAIR-1在巨核细胞系上的表达,探讨其表达与巨核细胞分化发育的关系,为深入研究LAIR-1在巨核细胞造血中的作用奠定基础。分别通过RT-PCR技术和流式细胞术在核酸和蛋白质水平上检测LAIR-1在巨核细胞系HEL、Dami、Mo7e中的表达。应用PMA和TPO刺激Dami细胞分化,流式细胞术和激光共聚焦显微镜技术检测Dami细胞分化过程中CD41a、CD42b和LAIR-1分子的表达。结果显示:Dami细胞中LAIR-1分子在核酸水平上呈现高表达,但膜蛋白表达水平很低。在PMA诱导Dami细胞分化过程中LAIR-1分子膜表达水平显著上调,PMA未刺激的Dami细胞中LAIR-1分子的阳性表达率为2.78%±0.50%,PMA刺激6 d后,Dami细胞中LAIR-1分子的阳性表达率为49.52%±1.86%。PMA可显著诱导Dami细胞中LAIR-1分子的表达;Dami细胞的分化与LAIR-1分子的表达水平之间存在因果关系;由于PMA是PKC的激活剂,LAIR-1分子表达水平可能受PKC信号通路的调节。
To study the expression of LAIR-1 on megakaryocyte and explore the relationship between the expression of megakaryocyte and the differentiation of megakaryocytic cells, which will lay the foundation for the further study on the role of LAIR-1 in hematopoietic cells of megakaryocytes. The expression of LAIR-1 in the megakaryocytic line HEL, Dami, Mo7e was detected by RT-PCR and flow cytometry at the nucleic acid and protein levels, respectively. Dami cell differentiation was stimulated by PMA and TPO, and the expression of CD41a, CD42b and LAIR-1 molecules in Dami cells was detected by flow cytometry and laser confocal microscopy. The results showed that the LAIR-1 molecule in Dami cells was highly expressed at the nucleic acid level, but the membrane protein expression level was very low. The expression of LAIR-1 was significantly up-regulated in Dami cells induced by PMA. The positive expression rate of LAIR-1 in Dami cells without PMA stimulation was 2.78% ± 0.50%. After DMA stimulation for 6 days, LAIR- -1 molecule positive expression rate of 49.52% ± 1.86%. PMA can significantly induce the expression of LAIR-1 in Dami cells. There is a causal relationship between the differentiation of Dami cells and the expression level of LAIR-1. Because PMA is an activator of PKC, the expression level of LAIR-1 may be affected by PKC signal Path adjustment.