目的比较树突状细胞(DC)以两种不同方式负载大肠癌细胞株体外刺激淋巴细胞的抗瘤活性。方法分离正常人外周血单核细胞体外诱导DC,分别负载热休克诱导凋亡的大肠癌细胞株和肿瘤细胞裂解物,以此刺激淋巴细胞作为效应细胞,大肠癌细胞株为靶细胞。MTT法测定效应细胞对靶细胞的杀伤作用。结果负载热休克大肠癌细胞的DC与负载肿瘤细胞裂解物的DC都显示对靶细胞的杀伤活性,但前者的杀伤率明显高于后者(P<0.05)。结论负载热休克肿瘤细胞的DC是一更为有效的负载方式。 Objective To compare the anti-tumor activity of dendritic cells (DCs) loaded with colorectal cancer cell lines in vitro in two different ways to stimulate lymphocytes. METHODS: DCs were isolated from normal human peripheral blood mononuclear cells in vitro. Heat-shock-induced colorectal cancer cell lines and tumor cell lysates were induced to stimulate lymphocytes as effector cells and colorectal cancer cell lines as target cells. The killing effect of the effector cells on the target cells was measured by MTT assay. Results DCs loaded with heat shock colorectal cancer cells and DCs loaded with tumor cell lysates showed killing activity against the target cells, but the killing rate of the former was significantly higher than that of the latter (P<0.05). Conclusion DCs loaded with heat shock tumor cells are a more effective loading method.