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目的 :观察短期暴露于不同剂量细颗粒物(fine particulate matter,PM2.5)对哮喘小鼠气道炎症的影响,并初步探索PM2.5影响哮喘小鼠气道炎症机制。方法:将40只Balb/c小鼠采用随机数字表法分为5组:对照组、卵清蛋白(ovalbumin,OVA)组、OVA+低剂量PM2.5(10μg)组、OVA+中剂量PM2.5(31.6μg)组和OVA+高剂量PM2.5(100μg)组。通过腹腔注射OVA致敏、雾化吸入OVA构建小鼠哮喘模型,第26、28、30天予以PM2.5滴鼻激发。第31天处死小鼠后观察HE染色观察各组小鼠肺组织病理变化、炎性细胞浸润情况,比较各组小鼠肺泡灌洗液(BALF)中细胞总数及分类计数;并通过ELISA方法检测各组小鼠肺泡灌洗液中IL-3、IL-14及血清Ig E水平。结果:OVA+低剂量PM2.5组小鼠BALF中细胞总数、分类计数百分比及IL-4、IL-13水平与OVA组相比均无统计学差异(P>0.05);而OVA+中剂量PM2.5组及OVA+高剂量PM2.5组较OVA组升高(P<0.05)。OVA+低剂量PM2.5组和OVA+中剂量PM2.5组血清Ig E与OVA组相比轻度升高,但无统计学差异(P>0.05);OVA+高剂量PM2.5组较OVA组升高(P<0.05)。结论:中剂量PM2.5(31.6μg)和高剂量PM2.5(100μg)可进一步加重哮喘小鼠气道炎症;而低剂量PM2.5(10μg)对哮喘小鼠气道炎症无显著影响。
OBJECTIVE: To observe the effect of short-term exposure to airway inflammation in mice with different doses of PM2.5 and to explore the mechanism of PM2.5 affecting airway inflammation in asthmatic mice. Methods: 40 Balb / c mice were randomly divided into 5 groups: control group, ovalbumin (OVA) group, OVA + low dose PM2.5 (10μg) group, OVA + medium dose of PM2.5 (31.6μg) group and OVA + high dose PM2.5 (100μg) group. The mice were sensitized by intraperitoneal injection of OVA and the asthma model was induced by inhalation of OVA by atomization. PM2.5 nasal challenge was given on the 26th, 28th and 30th days. The mice were sacrificed on the 31st day. The pathological changes and infiltration of inflammatory cells in the lung tissue of the mice were observed by HE staining. The total number of cells in the bronchoalveolar lavage fluid (BALF) and the number of the mice in each group were compared. The levels of IL-3, IL-14 and IgE in bronchoalveolar lavage fluid of mice in each group. Results: There was no significant difference in BALF between OVA + low dose PM2.5 group and the OVA group (P> 0.05). However, there was no significant difference in the number of BALF between OVA + low dose PM2.5 group and the OVA group (P> 0.05). 5 group and OVA + high dose PM2.5 group than OVA group (P <0.05). OVA + low dose PM2.5 group and OVA + medium dose PM2.5 group serum Ig E slightly increased compared with OVA group, but no significant difference (P> 0.05); OVA + high dose PM2.5 group than OVA group High (P <0.05). CONCLUSION: PM2.5 (31.6μg) and PM2.5 (100μg) at high dose can further aggravate airway inflammation in asthmatic mice, whereas PM2.5 at low dose (10μg) has no significant effect on airway inflammation in asthmatic mice.