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以一个栽培稻（ＯｒｙｚａｓａｔｉｖａＬ．ｓｐ．ｉｎｄｉｃａ）和野生稻（Ｏ．ｒｕｆｉｐｏｇｏｎＧｒｉｆ）杂交的Ｆ２作图群体以及由该群体构建的ＲＦＬＰ标记连锁图，分析了微卫星ＤＮＡ和ＡＦＬＰ标记的多态性、遗传行为及其在染色体上的分布。共定位了２８个微卫星ＤＮＡ标记和１７２个ＡＦＬＰ标记。２８个微卫星ＤＮＡ标记中有６个为华中农业大学作物遗传改良国家重点实验室根据数据库中序列而设计，其余２２个来自美国Ｃｏｒｎｅｌ大学已发表的结果。１７２个ＡＦＬＰ标记出自２５对引物扩增得到的２２８个多态性带的片段。这些标记分布于水稻的１２条染色体。将此２００个ＰＣＲ标记与华中农业大学作物遗传改良国家重点实验室构建的ＲＦＬＰ连锁图整合，得到一张含６１２个分子标记位点的遗传连锁图。
The F2 mapping population crossed with Oryzasativa L. sp. Indica and O. rufipogon Grif and the RFLP linkage map constructed from this population were analyzed for microsatellite DNA and AFLP marker polymorphism, Behavior and its distribution on chromosomes. Altogether 28 microsatellite DNA markers and 172 AFLP markers were located. Six of the 28 microsatellite DNA markers were designed by the State Key Laboratory of Crop Genetic Improvement at Huazhong Agricultural University based on the sequences in the database and the remaining 22 were published by Cornel University in the United States. 172 AFLP markers were from fragments of 228 polymorphic bands amplified by 25 pairs of primers. These markers are located in rice’s 12 chromosomes. The 200 PCR markers were integrated with the RFLP linkage map constructed by the State Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University to obtain a genetic linkage map containing 612 molecular marker loci.