论文部分内容阅读
致病酵母是条件致病菌感染中最常见的菌群。其属间、种间及种内的分型具有重要的流行病学及临床意义。以随机扩增多态性(Randomly Amplified Polymorphism DNA markers,RAPD)的方法对48株临床上常见的酵母菌属间、种间及种内基因组型的多态性进行了研究,并以多种引物扩增带型的相似性系数的高低来评价酵母菌之间的亲缘关系。结果表明:RAPD带型可清楚的显示出假丝酵母(Candida)及相关酵母属间、种间及种内的差异,亲缘关系的研究表明假丝酵母属与隐球菌属(Cryptococcus)、丝孢酵母属(Trichosporon)的相似性系数为80％,除季也蒙假丝酵母(C. guilliermondii)外,假丝酵母属中不同种间的相似性系数为82％～87％,同种不同株间的相似性系数>90％。大多数属、种基因组分型的结果和形态学分类结果相符。
Pathogenic yeast is the most common bacterial infection in opportunistic pathogens. Their genus, interspecific and intraspecific classification has important epidemiological and clinical significance. A total of 48 clinically common polymorphisms of yeast genus, interspecies and intraspecific genotypes were studied by Randomly Amplified Polymorphism DNA markers (RAPD) Amplification band similarity coefficient to evaluate the relationship between yeasts. The results showed that the RAPD patterns clearly showed the differences between Candida and related Saccharomyces, interspecific and intraspecies. The genetic relationship between Candida and Cryptococcus, The similarity coefficient of Trichosporon was 80%, except Candida albicans (C. guilliermondii), Candida species in different species similarity coefficient of 82% to 87%, the same kind of different strains The similarity coefficient between> 90%. Most genera and species genotyping results are consistent with morphological classification results.