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目的:比较放射免疫法和高效液相色谱(HPLC)法检测尿白蛋白浓度的差异,探讨高效液相色谱(HPLC)法在原发性IgA肾病(IgAN)的预后和随访中的应用价值。方法:对经肾病理确诊为原发性IgAN患者41例行HPLC法尿白蛋白检测,与放射免疫法检测尿白蛋白结果比较分析。结果:在原发性IgA肾病患者尿中,存在免疫化学非反应性白蛋白。采用HPLC法能检出更多的尿白蛋白,是放射免疫法结果的1.1～5.1倍。结论:HPLC法比放射免疫法能检出更多的尿白蛋白,提示尿白蛋白高效液相色谱(HPLC)法检测可用于原发性IgAN的长期、动态随访,并有助于评估肾病预后。
OBJECTIVE: To compare the differences of urinary albumin concentrations between radioimmunoassay and high performance liquid chromatography (HPLC), and to explore the value of high performance liquid chromatography (HPLC) in the prognosis and follow-up of primary IgA nephropathy. Methods: 41 cases diagnosed as primary IgAN by renal pathology were detected by HPLC urinary albumin, and radioimmunoassay was compared with urine albumin. Results: In the urine of patients with primary IgA nephropathy, immunochemical non-reactive albumin is present. Using HPLC method to detect more urinary albumin, radioimmunoassay results 1.1 to 5.1 times. Conclusion: Compared with radioimmunoassay, HPLC method can detect more urinary albumin, suggesting that urinary albumin high performance liquid chromatography (HPLC) can be used for long-term and dynamic follow-up of primary IgAN and to evaluate the prognosis of renal disease .