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应用聚合酶链反应（PCR）技术检测了60例老年慢性阴塞性肺病（COPD）患者的临床标本（咽部分泌物）．结果发现10例肺炎支原体（MP）阳性，同时对这10例阳性者用酶联免疫法（ELISA）检测其血清抗体予以对照，发现4例为阴性．提示MP感染是COPD急性发作的重要原因之一，而PCR技术可以快速、灵敏地检测COPD急性发作时MP感染，其检出率高于用ELISA法．
The clinical samples (pharyngeal secretions) of 60 elderly patients with chronic obstructive pulmonary disease (COPD) were detected by polymerase chain reaction (PCR). The results showed that 10 cases of Mycoplasma pneumoniae (MP) positive, at the same time these 10 cases were detected by enzyme-linked immunosorbent assay (ELISA) serum antibodies were compared and found that 4 were negative. It is suggested that MP infection is one of the important causes of acute exacerbation of COPD. However, PCR can detect MP infection rapidly and sensitively in acute exacerbation of COPD, and its detection rate is higher than that of ELISA.