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目的探讨趋化因子ENA-78(epithelial neutrophil activating peptide 78,ENA-78)在重症急性胰腺炎(acutepancreatitis,SAP)发病过程中的作用及姜黄素(curcumin,CUR)的干预效应。方法 54只SD大鼠按完全随机法分为SAP组、姜黄素治疗(SAP+CUR)组和对照组,用4%牛磺胆酸钠逆行胆胰管注射建立大鼠模型,SAP+CUR组在建立模型前2 h腹腔内注射姜黄素溶液(200 mg/kg),余组注射同等剂量的DMSO溶液。用ELISA法测定3组大鼠不同时点血清中ENA-78水平的变化;Western blot和免疫组化法检测胰腺组织不同时点ENA-78蛋白的表达水平;病理组织切片检查各时点胰腺的病理变化。结果 SAP组血清中ENA-78水平随时间的延长逐渐增强,3 h后各时点血清ENA-78水平均高于对照组及SAP+CUR组(P<0.01);SAP组及SAP+CUR组术后胰腺组织ENA-78蛋白表达随时间延长逐渐增加,术后各时间点表达均显著高于对照组,但同时间点比较SAP+CUR组表达明显低于SAP组(P<0.01);病理组织切片检查显示SAP+CUR组各时点与SAP组相比较,胰腺病理损伤显著减轻。结论姜黄素能有效干预SAP,其机制可能与抑制趋化因子ENA-78的表达有关。
Objective To investigate the role of epithelial neutrophil activating peptide 78 (ENA-78) in the pathogenesis of severe acute pancreatitis (SAP) and the intervention effect of curcumin (CUR). Methods 54 SD rats were randomly divided into SAP group, curcumin treatment group and control group. Rats in SAP + CUR group were treated with 4% sodium taurocholate retrograde cholangiopancreatography. The curcumin solution (200 mg / kg) was intraperitoneally injected 2 h before the model was established, and the rest group was injected with the same dose of DMSO solution. The levels of ENA-78 in the serum of the three groups of rats at different time points were measured by ELISA. The expression of ENA-78 protein in the pancreas at different time points was detected by Western blot and immunohistochemistry. The histopathological sections of the pancreas Pathological changes. Results The level of ENA-78 in serum of SAP group increased gradually with the prolongation of time. The level of serum ENA-78 in SAP group was higher than that in control group and SAP + CUR group at 3 hours (P <0.01) The expression of ENA-78 protein in pancreatic tissue increased gradually with time, and the expression of ENA-78 protein in pancreatic tissue at each time point was significantly higher than that in control group. However, the expression of ENA-78 protein in SAP group was significantly lower than that in SAP group at the same time point (P <0.01) Tissue biopsy showed that SAP + CUR group at each time point compared with the SAP group, pancreatic pathological damage significantly reduced. Conclusion Curcumin effectively interferes with SAP, and its mechanism may be related to the inhibition of the expression of chemokine ENA-78.