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作者首次报道将聚合酶链反应（ＰＣＲ）技术用于检测非核酸物质－胃癌相关抗原，建立了免疫－ＰＣＲ技术。首先制备胃癌单抗及重组ＤＮＡ的嵌合体即ＭＧ７—ｐＸＪ１９，以将抗原抗体反应的专一性同ＰＣＲ技术的高敏感性相结合，构建一种集ＡＢＣ及ＰＣＲ一体的双重放大系统。利用这种技术检测胃印戒细胞癌株ＫＡＴＯⅢ肿瘤相关抗原，并与ＥＬＩＳＡ法比较其敏感性。结果表明应用免疫－ＩＣＲ技术ＫＡＴＯⅢ细胞数目在２０个时即可出现阳性结果，敏感性较ＥＬＩＳＡ法高出１０４倍。若以０．２５％戊二醛固定相同数量（２×１０６／ｍｌ）ＫＡＴＯⅢ细胞后加入不同浓度的ＭＧ７－ｐＸＪ１９嵌合体，则免疫－ＰＣＲ检测结果呈阳性时所需ＭＧ７－ｐＸＪ１９为３．８×１Ｏ－１４ｍｏｌ，而ＥＬＩＳＡ法需３．０×１０－１１ｍｏｌ。研究结果表明，免疫－ＰＣＲ技术可用于肿瘤相关抗原的检测，具有高敏感性，高特异性，结果稳定且重复性满意。
The authors reported for the first time that the polymerase chain reaction (PCR) technique was used to detect non-nucleic acid substances-gastric cancer-associated antigens and established an immuno-PCR technique. Firstly, the gastric cancer monoclonal antibody and recombinant DNA chimera, MG7-pXJ19, were prepared to combine the specificity of antigen-antibody reaction with the high sensitivity of PCR technology to construct a double amplification system integrating ABC and PCR. This technique was used to detect the KATOIII tumor-associated antigen of gastric signet ring cell carcinoma and compare its sensitivity with ELISA. The results showed that the application of immuno-ICR technology KATOIII cell number can be positive when the 20 results, the sensitivity of 104 times higher than the ELISA method. If the same amount (2×10 6 /ml) of KATO III cells was fixed in 0.25% glutaraldehyde and different concentrations of MG7-pXJ19 chimera were added, the MG7-pXJ19 required for immuno-PCR was 3.8. ×1O-14 mol, whereas the ELISA method requires 3.0×10-11 mol. The results showed that the immuno-PCR technique can be used for the detection of tumor-associated antigens, with high sensitivity, high specificity, stable results and satisfactory repeatability.