[Objective]This study aimed to establish an appropriate technology system for in vitro screening of Fusarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid,thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermelon. [Method] Using Zhongxin No. 1 red edible seed watermelon adventitious buds as screening materials and fusaric acid( FA) as a stress agent,in vitro screening of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [Result]The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon variants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenerated plants were obtained. Among the 36 germplasm resources of red edible seed watermelon,there were 2 highly resistant materials,6 moderately resistant materials,11 slightly resistant materials and 17 highly susceptible materials. [Conclusion]This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon. [Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fusarium wilt-resistant germplasm resources of red edible seed watermelon and access variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermelon [Method] Using Zhongxin No. 1 red edible seed watermelon adventitious buds as screening materials and fusaric acid (FA) as a stress agent, in vitro screening of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon variants was 15 mg / L. In vitro screening system for Fusarium wilt- resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenerated plants were germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materials, 11 slightly resistant materials and 17 highly susceptible materials. [Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon.