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采用高效液相色谱法建立了白芷水提液指纹图谱,色谱柱为Lichrospher 5-C18(250 mm×4.6 mm,5μm),以甲醇-水为流动相进行二元梯度洗脱,柱温30℃,流速为1 mL/min,检测波长为254 nm。对12批不同产地的白芷药材进行了检测,共发现13个共有色谱峰,各色谱峰分离情况良好。精密度、重复性和稳定性试验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,符合指纹图谱要求。该方法简便、可靠,精密度、稳定性和重复性良好,可用于白芷药材的质量评价。
The HPLC fingerprint of Angelica dahurica was established. The chromatographic column was Lichrospher 5-C18 (250 mm × 4.6 mm, 5 μm) with a binary gradient elution with methanol-water as the mobile phase. The column temperature was 30 ℃ , The flow rate was 1 mL / min and the detection wavelength was 254 nm. 12 batches of different areas of Angelica herbs were detected, found a total of 13 common peaks, the chromatographic peak separation is good. The RSDs of the relative retention time and the relative peak area of each common peak in the precision, repeatability and stability test were all less than 3%, which accorded with the fingerprinting requirements. The method is simple, reliable, good precision, stability and repeatability, and can be used for the quality evaluation of Angelica dahuricae.