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目的 了解AgNOR定量分析与PCNA基因表达在评判胃癌预后中的意义。 方法 对 80例胃癌石蜡标本进行HE染色分组 ,分别进行AgNOR染色及S P免疫组化方法染色。应用MPILAS 5 0 0多媒体彩色图分析系统进行AgNOR定量分析和光镜下观察PCNA阳性表达结果。 结果 AgNOR含量在进展期组与早期组相比差异有显著意义 (P <0 0 1) ,生存时间≤ 3年组与生存时间≥ 3年组相比差异有显著意义 (P <0 0 1)。结论 AgNOR定量分析与PCNA基因表达是胃癌较强的预后指标。联合检测定性、定量指标并综合分析能更加有效地评判病人预后。
Objective To understand the significance of AgNOR quantitative analysis and PCNA gene expression in the evaluation of gastric cancer prognosis. Methods 80 cases of gastric cancer paraffin specimens were HE staining group, respectively, AgNOR staining and SP immunohistochemical staining. The MPILAS 500 color image analysis system was used for AgNOR quantitative analysis and PCNA positive expression under light microscope. Results There was significant difference in the AgNOR content between the advanced stage group and the early stage group (P <0.01), the survival time less than 3 years group and the survival time> 3 years group had significant difference (P <0.01) . Conclusion AgNOR quantitative analysis and PCNA gene expression is a strong prognostic indicator of gastric cancer. Joint detection of qualitative, quantitative indicators and comprehensive analysis can more effectively judge the patient’s prognosis.