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目的:检测急性髓性白血病患者外周血T淋巴细胞亚群的变化及CD56+细胞比例的临床意义。方法:选取确诊但未治疗的急性白血病30例为初治组;确诊并经化疗达完全缓解的急性白血病30例为复治组;健康志愿者30例为正常对照组。用流式细胞仪检测初治组、复治组及对照组人群的外周血T淋巴细胞亚群及CD56+细胞的比例,检测初治组中不同CD56+细胞组间患者血红蛋白及白蛋白水平。结果:初治组的CD3+T淋巴细胞、CD4+T淋巴细胞、CD8+T淋巴细胞、CD4+/CD8+比值较复治组及对照组均有显著性下降(P<0.05),而复治组与对照组比较差别无统计学意义(P>0.05)。初治组AML的CD56+细胞比例超过25%患者为40%(12/30),而复治组仅10%(3/30),差别具有统计学意义(P<0.05)。初治组中CD56+细胞高表达组的血红蛋白(Hb)及白蛋白(Alb)显著性低于正常表达组(P<0.05)。结论:初治组急性髓性血病患者的T淋巴细胞亚群变化明显,说明细胞免疫与白血病的发生、发展及转归有关;急性髓性白血病患者CD56高表达提示临床状况较差,生活质量低,可能与预后不良有关。
Objective: To detect the changes of T lymphocyte subsets and the proportion of CD56 + cells in peripheral blood of patients with acute myeloid leukemia. Methods: Thirty patients with confirmed and untreated acute leukemia were selected as the naive group. Thirty patients with acute leukemia who were diagnosed as having complete remission by chemotherapy were treated by Fujii group and 30 healthy volunteers were normal control group. The proportion of T lymphocyte subsets and CD56 + cells in the peripheral blood of the newly diagnosed, retarcted and control groups were detected by flow cytometry. The levels of hemoglobin and albumin in the patients with CD56 + Results: The CD3 + T lymphocytes, CD4 + T lymphocytes, CD8 + T lymphocytes and CD4 + / CD8 + ratio of the newly diagnosed group were significantly lower than those of the retreatment group and the control group (P <0.05) Compared with the control group, the difference was not statistically significant (P> 0.05). The rate of CD56 + cells in the AML group was 40% (12/30) in the AML group and only 10% (3/30) in the AML group. The difference was statistically significant (P <0.05). In newly diagnosed group, the expression of Hb and Alb in CD56 + cells was significantly lower than that in normal cells (P <0.05). Conclusion: The T lymphocyte subsets of patients with acute myeloid leukemia in the newly diagnosed group changed obviously, indicating that the cellular immunity is related to the occurrence, development and prognosis of leukemia. The high expression of CD56 in patients with acute myeloid leukemia suggests that the clinical condition is poor, and the quality of life Low, may be related to poor prognosis.