电针镇痛的同时,大鼠皮层、尾核、丘脑和脑干网状结构内的乙酰胆碱含量都明显的升高。为了进一步证明乙酰胆碱在针刺镇痛中的作用和地位,本实验观察了针刺镇痛对黄牛脑脊液内乙酰胆碱含量的影响。 实验方法 选用本地役用成年黄牛22头,其中雄性6头,雌性16头。侧卧保定,用剪刀在测痛部位剪毛并用酒精、乙醚脱去皮脂,然后涂以10％氯化钾溶液,再将二电极与皮肤紧密 At the same time as electroacupuncture analgesia, the content of acetylcholine in the rat cortex, caudate nucleus, thalamus, and brainstem reticular formation increased significantly. In order to further prove the role and status of acetylcholine in acupuncture analgesia, this experiment observed the effect of acupuncture analgesia on the content of acetylcholine in cerebrospinal fluid of cattle. Experimental methods 22 domestic adult cattle were selected for use, including 6 males and 16 females. On the side of Baoding, use scissors to cut hair at the site of pain measurement and remove sebum with alcohol and ether, then apply 10% potassium chloride solution, and then close the two electrodes to the skin.