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目的：：建立维地红涂剂中地塞米松的含量测定方法,保障临床用药安全有效。方法：高效液相色谱法,迪马C18柱(150 mm ×4.6 mm,5μm)；流动相为甲醇-水-冰醋酸(80∶20∶0.5)；检测波长：240 nm；进样量：20μl；柱温：30℃；流速：1 ml· min-1。结果：地塞米松在3.0~96.0μg·ml-1范围内线性关系良好(r=0.9997),平均回收率为99.45%,RSD=1.01%(n=9)。结论：所建方法便捷、重复性好,适用于维地红涂剂中地塞米松的含量测定。“,”Objective:To establish a method for the determination of dexamethasone in Verdihong paints to ensure safety and ef-fectiveness of the clinical medicines. Methods:An HPLC method with a Dikma C18 column (150 mm × 4. 6 mm, 5 μm) was used, the mobile phase was a mixture of methanol-water-glacial acetic acid (80∶20∶0. 5), the detection wavelength was at 240nm, the injec-tion volume was 20 μl, the column temperature was 30℃ and the flow rate was 1. 0 ml·min-1 . Results:The standard curve of dexa-methasone was linear over the range of 3. 0-96. 0μg·ml-1(r=0. 999 7). The average recovery was 99. 45% with RSD of 1. 01%(n=9). Conclusion:The method is simple with good reproducibility, and can be used in the determination of dexamethasone in Verdihong paints.