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孢子萌发及附着胞形成是昆虫病原真菌侵染寄主过程中的关键步骤.观察了绿僵菌CQMa102菌株孢子在蝗虫后翅上萌发及附着胞形成率、芽管及附着胞形态情况.结果表明,在蝗翅上培养8h后孢子开始萌发,附着胞通常培养16h时出现,20～36h内大量生成.营养丰富有利于孢子萌发,但不利于附着胞形成.与用玻璃作基质相比,蝗翅有利于诱导附着胞形成,且蝗翅上绿僵菌附着胞个体较大,芽管短,分枝少,隔膜少.
Spore germination and attachment formation are the key steps in insect host pathogen infestation.We observed the germination and the formation rate of adherent cells and the morphology of adherent cells and spores of spores of Metarhizium anisopliae strain CQMa102.The results showed that, After cultured for 8h on the locusts, the spores began to germinate, and the attachment cells usually appeared after 16h culture, and were abundantly produced within 20-36h. Nutrient enrichment was beneficial to spore germination, but not conducive to the formation of adherent cells. Is conducive to induce the formation of attachment cells, and Metarhizium anisopliae attachment cells on a large, germinated tube short, fewer branches, fewer septum.