论文部分内容阅读
目的观察氯硝柳胺原位固化长效注射剂对感染日本血吸虫(Schistosoma japonicum)的小鼠和水牛的疗效。方法 50只昆明小鼠随机均分为2组,给药组小鼠皮下注射低浓度氯硝柳胺原位固化长效注射剂(71 mg/ml)450 mg/kg 1次,对照组小鼠不作任何处理。给药后第1、15、43、57和71天,各组分别取5只小鼠采用腹部贴片法感染日本血吸虫尾蚴(80±4)条/鼠。35 d后,处死小鼠,检获成虫,计算减虫率。10头水牛分成2组,给药组4头、对照组6头。给药组水牛皮下注射高浓度氯硝柳胺原位固化长效注射剂(222 mg/ml)30 mg/kg1次,对照组水牛不作任何处理。于给药后第1和3个月粪检,计算粪检阳性率,并观察水牛的不良反应。采用SPSS19.0软件进行统计学分析,组间比较采用t检验。结果给药后第1、15、43、57和71天感染日本血吸虫尾蚴,35 d后给药组小鼠的虫荷分别为(3.6±1.4)条、(17.5±8.6)条、(10.0±6.8)条、0条、(11.3±8.4)条,均少于对照组小鼠的虫荷(P<0.05),且减虫率均超过50.0%。第1和3个月对水牛进行粪检,给药组均为阴性,对照组分别有2和3头阳性,其中1头牛给药后8 h出现精神沉郁。结论氯硝柳胺原位固化长效注射剂预防小鼠感染日本血吸虫尾蚴的作用达2个月,预防水牛感染日本血吸虫尾蚴的作用达3个月,具有现场应用预防血吸虫感染的潜力。
Objective To observe the curative effect of niclosamide in situ curing long-acting injection on mice infected with Schistosoma japonicum and buffaloes. Methods Fifty Kunming mice were randomly divided into two groups. The mice in the control group were injected subcutaneously with 450 mg / kg niclosamide (450 mg / kg) Any deal. On days 1, 15, 43, 57 and 71 after administration, 5 mice in each group were infected with cercariae of Schistosoma japonicum (80 ± 4) / mouse by abdominal patch method. After 35 days, the mice were sacrificed and adult worms were seized to calculate the worm reduction rate. Ten buffaloes were divided into two groups, four for the administration group and six for the control group. The buffalo in the treatment group were subcutaneously injected with 30 mg / kg long-acting injection of niclosamide (222 mg / ml) in high concentration for 1 time. The buffaloes in the control group were not given any treatment. Fecal seizures were performed on the first and third months after administration, and the positive rate of fecal examination was calculated, and the adverse reactions of buffaloes were observed. SPSS19.0 software was used for statistical analysis, t-test was used to compare between groups. Results The infection rates of cercariae of Schistosoma japonicum on the 1st, 15th, 43th, 57th and 71 days after administration were (3.6 ± 1.4), (17.5 ± 8.6) and (10.0 ± 6.8), 0 and (11.3 ± 8.4) were less than those in the control group (P <0.05), and the worm reduction rates were over 50.0%. Buffaloes were tested in the first and third months, all of which were negative in the treatment group, and 2 and 3 were positive in the control group, respectively. One of the cattle showed depression after 8 hours of administration. Conclusion Niclosamide in situ curing long-acting injection can prevent mice infected with Schistosoma japonicum cercariae for 2 months and prevent the water buffalo from inoculating with cercariae of Schistosoma japonicum for 3 months. It has the potential to prevent Schistosoma japonicum infection in the field.