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目的比较研究新疆维吾尔自治区与甘肃省部分地区结核分枝杆菌分子流行病学特征。方法 2008年3-12月从新疆维吾尔自治区与甘肃省的3个县(区)收集痰涂片阳性的结核病患者的临床分离株。收集患者的性别、民族、病史、菌株耐药谱和菌株来源资料。药物敏感性实验采用比例法。运用实时荧光定量PCR(RealtimePCR)和结核分枝杆菌散在分布重复单位(MIRU-VNTR)基因分型方法鉴定北京基因型及亚型菌株并绘制系统发育树。结果在146份结核分枝杆菌分离株中,北京/W系结核分枝杆菌84株,占57.5%。其中较现代的W菌/典型北京家族菌株66株,占78.6%。MIRU-VNTR基因分型方法将146株菌株分成140个基因型,其中包括134个独特型,6个成簇的基因型。结论在甘肃采样地区,北京/W系的流行呈现明显优势,其中较为现代的W菌/典型北京家族菌株的流行又呈较为明显的优势;而在新疆采样地区,以非北京/W系菌株流行为主,两地结核分枝杆菌分子流行病学特征有明显区别。
Objective To compare the molecular epidemiological characteristics of Mycobacterium tuberculosis between Xinjiang Uygur Autonomous Region and parts of Gansu Province. Methods From March to December 2008, clinical isolates of sputum smear-positive TB patients were collected from 3 counties (districts) in Xinjiang Uygur Autonomous Region and Gansu Province. Collect the patient’s gender, ethnicity, medical history, strains of drug resistance spectrum and strain source information. Drug sensitivity experiments using the proportion method. Real-time PCR (Realtime PCR) and MIRU-VNTR genotyping were used to identify Beijing genotypes and subtypes and phylogenetic tree was constructed. Results Of the 146 M. tuberculosis isolates, 84 strains of Mycobacterium tuberculosis were Beijing / W strains, accounting for 57.5%. Among them, 66 were W type / typical Beijing family strains, accounting for 78.6% of the total. The MIRU-VNTR genotyping method divides 146 strains into 140 genotypes, including 134 idiotypes and 6 clustered genotypes. Conclusions The prevalence of Beijing / W series in Gansu sampling areas showed obvious advantages. The prevalence of the more modern W bacteria / typical Beijing family strains showed obvious advantages. However, in the sampling areas of Xinjiang, the prevalence of non-Beijing / W strains Mainly the molecular epidemiology of Mycobacterium tuberculosis between the two regions have obvious differences.