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高分辨率是光学显微技术发展至今不断追求的目标之一。然而随着当前显微镜系统功能与性能的不断革新,高分辨率与大视场难以同时兼顾的问题日益突出,这个问题极大地限制了其在许多领域的应用。傅里叶叠层显微成像技术(FPM)是近年来发展出的一种新型计算成像技术,其能通过同时恢复强度和相位分布来提供宽域高分辨率的成像能力。FPM虽然是在2013年才被提出,但是由于其融合了大视场、高分辨率、定量相位成像等诸多优点,近年来已经在光学显微、生物医学、生命科学等领域获得了大量研究和广泛关注。从基本原理、实验系统与成像模式、系统与算法的改进方法等几个方面对FPM的研究现状、应用领域和最新进展进行了综述,并讨论了现存的一些关键问题以及今后可能的研究方向。
High resolution is one of the goals pursued by the development of optical microscopy. However, with the continuous innovation of the function and performance of the current microscope system, the problem that the high resolution and the large field of view are difficult to take into account simultaneously has become increasingly prominent. This problem greatly limits its application in many fields. Fourier stack microscopy (FPM) is a new computational imaging technique developed in recent years that provides wide-area, high-resolution imaging capabilities by simultaneously restoring intensity and phase distribution. Although FPM was proposed only in 2013, FPM has obtained a great deal of research in optical microscopy, biomedicine, life science and other fields in recent years due to its merits of large field of view, high resolution and quantitative phase imaging extensive attention. The research status, application fields and latest progress of FPM are reviewed from the aspects of basic principle, experimental system and imaging mode, and the improvement of system and algorithm. Some existing key problems and the possible future research directions are also discussed.