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目的 探讨胎儿卵巢能否用于临床移植。方法 取自流产或病理原因水囊引产的胎儿卵巢共 49份。将卵巢切成碎块进行组织培养 ,培养后观察组织学变化 ,同时测定培养液中雌二醇 (E2 )浓度 ;取培养前后胎儿卵巢用免疫组化法检测FSH -Binding、LH -Binding、LH -R、HLA -ABC、HLA -DR抗原含量 ;观察冷冻保存后卵泡形态发育及E2 分泌。结果 胎儿卵巢在体外培养条件下 ,能进一步发育 ,而且能分泌E2 ;胎儿卵巢最早于 2 0周出现FSH -Binding、LH -Binding、LH -R阳性表达 ;胎儿卵巢HLA -ABC、HLA -DR抗原含量明显低于成人卵巢 ,经体外培养后HLA -DR抗原含量进一步降低 ;胎儿卵巢复苏培养后卵泡仍能进一步发育和产生E2 。结论 胎儿卵巢可用于卵巢移植 ,作为供体优于成人卵巢
Objective To investigate whether fetal ovary can be used in clinical transplantation. Methods Taken from abortion or pathological reasons of the fetus induced by a total of 49 fetal ovary. The ovary was cut into pieces for tissue culture. After culturing, histological changes were observed. The estradiol (E2) concentration in the culture fluid was also determined. Fetal ovaries before and after culture were examined by immunohistochemistry for FSH-Binding, LH-Binding, LH R, HLA-ABC, HLA-DR antigen content; observed after cryopreservation follicular morphology and E2 secretion. Results Fetal ovary could develop further under in vitro culture conditions and could secrete E2. Fetal ovary showed FSH-Binding, LH-Binding and LH-R as early as 20 weeks. Fetal ovary HLA-ABC and HLA-DR antigen Content was significantly lower than that of adult ovary, HLA-DR antigen content decreased further in vitro; follicles can still further develop and produce E2 after fetal ovary resuscitation and culture. Conclusion Fetal ovary can be used for ovarian transplantation, as a donor superior to adult ovary