论文部分内容阅读
目的:观察槟榔十三味丸对慢性应激抑郁模型大鼠行为学和脑前额叶皮质、海马单胺类神经递质及其代谢产物的影响,探讨其抗抑郁作用机制。方法:将Wistar雄性大鼠,根据蔗糖水消耗量及体质量随机分为正常对照组、模型组、氟西汀组、槟榔十三味丸低、中、高剂量组,每组10只。除正常对照组外,其余大鼠均采用慢性轻度不可预见性应激结合孤养方法制备抑郁模型,造模同时灌胃给药,每日1次,连续给药21天。以敞箱实验、蔗糖水消耗实验进行行为学评价,并用高效液相-电化学法测定脑前额叶皮质、海马去甲肾上腺素(NE)、5-羟色胺(5-HT)、多巴胺(DA)及其代谢产物含量。结果:与正常对照组比较,模型组大鼠敞箱实验水平运动和垂直运动得分、蔗糖水消耗量显著降低(P<0.01或P<0.05);与模型组比较,槟榔十三味丸中、高剂量组和氟西汀组大鼠敞箱实验水平运动和垂直运动得分、蔗糖水消耗量均显著增加(P<0.05或P<0.01);高效液相-电化学法测定结果显示,模型大鼠脑前额叶皮质NE、5-HT、DA、DOPAC,海马5-HT、5-HIAA、DA含量较正常对照组显著下降(P<0.05或P<0.01),槟榔十三味丸可升高模型大鼠脑前额叶皮质NE、5-HT、DA、DOPAC和海马5-HT、5-HIAA、DA含量,尤其中、高剂量组作用显著(P<0.05或P<0.01)。结论:槟榔十三味丸能改善抑郁模型大鼠行为学异常,提高大鼠脑内神经递质NE、5-HT、DA及其代谢产物含量,这可能是槟榔十三味丸抗抑郁作用的重要机制之一。
OBJECTIVE: To observe the effects of betel nutteen serum on the behavior and the monoamine neurotransmitters and their metabolites in prefrontal cortex and hippocampus of rats with chronic stress depression, and to explore the antidepressant mechanism. Methods: Wistar male rats were randomly divided into normal control group, model group, fluoxetine group and betel nut low-fat, middle-dose and high-dose groups according to their sucrose water consumption and body weight. In addition to the normal control group, the rest of the rats were used chronic mild unpredictable stress combined with ligation methods to prepare depression model, while modeling intragastric administration, once a day, continuous administration of 21 days. The behavioral evaluation was carried out in open-field and sucrose-water consumption experiments. The levels of NE, 5-HT and DA in the prefrontal cortex, hippocampus, And its metabolites content. Results: Compared with the normal control group, the rats in the untreated group showed significantly lower level of open movement and vertical movement and sucrose water consumption (P <0.01 or P <0.05). Compared with the model group, High-dose group and fluoxetine group open-box experimental horizontal motion and vertical motion scores, sucrose water consumption were significantly increased (P <0.05 or P <0.01); high performance liquid-electrochemical assay showed that the model large The contents of NE, 5-HT, DA, DOPAC, 5-HT, 5-HIAA and DA in the prefrontal cortex of rats decreased significantly compared with the normal control group (P <0.05 or P <0.01) The levels of NE, 5-HT, DA, DOPAC and 5-HT, 5-HIAA and DA in the rat prefrontal cortex, especially middle and high dose groups, were significantly increased (P <0.05 or P <0.01). Conclusion: arecajisanweiwei pill can improve the behavioral abnormalities in depression model rats and increase neurotransmitters NE, 5-HT, DA and their metabolites in the rat brain, which may be the antidepressant effect of betel nut One of the important mechanisms.