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目的研究黄根乙酸乙酯部位对大鼠肝星状细胞(HSC-T6)的增殖抑制作用及其对相关细胞外基质的影响。方法采用MTT法检测黄根乙酸乙酯部位对大鼠肝星状细胞的增殖抑制并运用流式细胞术检测细胞凋亡率;采用酶联免疫吸附法(ELISA)检测细胞中TGF-β1、Co1-I及α-SMA蛋白表达。结果黄根乙酸乙酯部位对肝星状细胞的抑制作用随药物浓度的增长呈梯度增长;可诱导细胞发生早、中、晚期凋亡,各浓度凋亡率分别为2.5%、20.2%、43.9%;细胞中的TGF-β1、Co1-I、α-SMA蛋白,随药物浓度增加,其表达均下降,高剂量组(400 mg/L)与空白组相比具有显著性差异(P<0.05)。结论黄根乙酸乙酯部位在体外能抑制HSC-T6细胞增殖,其机制可能通过抑制细胞中TGF-β1、Co1-I、α-SMA蛋白表达,从而减少细胞外基质的合成与沉积,进而起到抗肝纤维化的作用。
Objective To study the inhibitory effect of ethyl acetate from Huanggen on the proliferation of rat hepatic stellate cells (HSC-T6) and its effect on the relevant extracellular matrix. Methods MTT assay was used to detect the inhibitory effect of ethyl acetate fraction on the proliferation of rat hepatic stellate cells and the apoptosis rate was detected by flow cytometry. The expressions of TGF-β1, Co1 -I and α-SMA protein expression. Results The inhibition of hepatic stellate cells by ethyl acetate fraction increased gradually with the increase of drug concentration. The apoptosis rate of early, middle and late apoptosis induced by ethyl acetate was 2.5%, 20.2% and 43.9 (P <0.05). The expression of TGF-β1, Co1-I and α-SMA in the high dose group (400 mg / L) were significantly lower than those in the blank group ). Conclusion The ethyl acetate fraction of Huanggen can inhibit the proliferation of HSC-T6 cells in vitro. The mechanism may be through the inhibition of the expression of TGF-β1, Co1-I and α-SMA in the cells, thereby reducing the synthesis and deposition of extracellular matrix To anti-liver fibrosis role.