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以6个燕麦品种为试材,设置不同的PEG胁迫强度,研究各燕麦品种主要生理指标的变化情况,以探究不同燕麦品种萌发期幼苗对PEG胁迫的生理适应机制。结果表明:不同燕麦品种幼苗的丙二醛含量随PEG胁迫强度的加重而不断累积,细胞膜损伤逐渐加重;抗氧化酶活性以及可溶性蛋白质(SP)含量在低浓度胁迫时逐渐增大,维持细胞内的渗透势,保护细胞膜免遭伤害,高浓度胁迫时表现为抑制作用,生理系统破坏严重;结合GGE双标图,发现不同燕麦品种适应PEG胁迫的作用生理指标不同:“甘早燕麦”通过SOD调节;“奥塔燕麦”和“青海444燕麦”由SP和MDA调节适应;“甜燕1号燕麦”通过POD、CAT调节;-0.4 MPa渗透胁迫环境具有较好的区分力和代表性,可作为燕麦生产的最佳胁迫强度和优良燕麦品种筛选的浓度。
Six oat cultivars were used as experimental materials to set up different PEG stress intensities. The main physiological indexes of oat cultivars were studied to investigate the physiological adaptation mechanisms of different oat cultivars to PEG stress. The results showed that the content of malondialdehyde in different oat varieties seedlings accumulated continuously with the increase of PEG stress, and the cell membrane injury gradually aggravated. The activities of antioxidant enzymes and soluble protein (SP) increased gradually at low concentration, Of the osmotic potential to protect the cell membrane from injury, the performance of high concentrations of stress inhibition, physiological system damage severely; combined with GGE double plot and found that different oats to adapt to different PEG physiological indicators of different physiological indicators: Regulated by SOD; “OTA” and “Qinghai 444 oat” were regulated by SP and MDA; “Sweetyan 1 oat” was regulated by POD and CAT; and -0.4 MPa osmotic stress had better environment The distinction and representativeness can be used as the optimal stress intensity for oat production and for the screening of good oats.