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细菌放射呼吸测量法是利用放射核素监测细菌生长、代谢的技术。也是一种专用于探测微生物代谢释放14CO2的技术。因为二氧化碳是许多重要生物反应的天然产物,所以通过对CO2的测定可以了解很多生物现象。尤其是细菌的体外培养几乎在代谢过程中都能放出CO2,因此培养基中加入14C-标记的营养物(如14C-葡萄糖、14C-氨基酸等),经细菌的代谢降解作用可生成14CO2气体,通过仪器测量放射性,可定量地检测细菌的数量。该类技术具有快速,敏感和客观等特点,用途较多。本研究试图在我们已建立的液体闪烁法细菌放射测定的基础上,观察需氧菌的培养和定量测定、水和食品中细菌总数的检测以及尿样品中细菌总数的检查等,以提供细菌放射测量法实际应用的参考。
Bacterial radiation respiratory measurement is the use of radionuclides to monitor bacterial growth and metabolism of technology. It is also a technique specifically designed to detect 14CO2 metabolism by microorganisms. Because carbon dioxide is a natural product of many important biological reactions, many biological phenomena can be understood by measuring CO2. In particular, in vitro bacterial culture releases CO 2 almost during metabolism. Therefore, by adding 14 C-labeled nutrients (such as 14 C-glucose, 14 C-amino acids, etc.) to the medium, 14 CO 2 gas can be produced by bacterial metabolism and degradation, By measuring radioactivity, the number of bacteria can be quantitatively measured. Such technology is fast, sensitive and objective characteristics, more uses. This study attempted to observe the culture and quantitative determination of aerobic bacteria, the total number of bacteria in water and foodstuffs, and the total number of bacteria in urine samples on the basis of the established liquid scintillation bacteriometry to provide bacterial radiation Measurement of practical reference.