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目的:研究香附四物汤全方水提液(QF)及其效应部位(BW)中3个生物碱类成分经口服给药后药物代谢动力学特征的差异。方法:采集比格犬灌胃QF及BW后不同时间点的血浆样本,采用LC-MS/MS以多反应离子监测(MRM)方式进行正离子检测,测定血浆中普鲁托品、延胡索乙素、四氢非洲防己碱的浓度,DAS软件计算主要药动学参数。结果:普鲁托品在QF与BW中的各项参数除Cmax外差异没有统计学意义,BW中Cmax大于QF(P<0.05);延胡索乙素在体内消除缓慢,QF和BW均出现了双峰现象,QF中Tmax、AUC0～T与BW相比明显增大(P<0.05);四氢非洲防己碱达峰较快,BW中Cmax大于QF(P<0.05)。结论:QF和BW药动学参数的差异有统计学意义,提示两者的差异成分会对普鲁托品、延胡索乙素和四氢非洲防己碱的体内过程产生影响。
OBJECTIVE: To study the differences of pharmacokinetics of three alkaloids in QF and its effect site (BW) after oral administration. Methods: Plasma samples from Beagle dogs were collected at different time points after QF and BW administration. Positive ions were detected by multi-reactive ion monitoring (MRM) with LC-MS / MS. The contents of Plutonium, , Tetrandrine concentration, DAS software to calculate the main pharmacokinetic parameters. Results: The parameters of Pluton in QF and BW had no significant difference except for Cmax, Cmax in BW was higher than that in QF (P <0.05), while Tetrahydropalmatine was slowly eliminated in vivo, and both QF and BW showed double Peak, Tmax and AUC0 ~ T in QF were significantly increased compared with BW (P <0.05). Tetrahydropalmatine peaked faster and Cmax in BW was higher than QF (P <0.05). CONCLUSIONS: The differences in pharmacokinetic parameters between QF and BW were statistically significant, suggesting that the differences between the two may have an effect on the in vivo processes of prutidine, tetrahydropalmatine and tetrandrine.