论文部分内容阅读
目的评价儿童幽门螺杆菌(Hp)的组织学免疫组化染色的诊断价值。方法对我院儿科就诊的102例具上消化道症状的患儿均行胃镜检查并取胃窦黏膜活检,分别经快速尿素酶(RUT)试验及组织免疫组化染色检测Hp;在胃镜检查前分别行13C-尿素呼气试验(13C-UBT)和现症感染条带(CIM)血清学检测Hp。以13C-UBT为诊断金标准,比较免疫组化与其他两种方法的敏感性、特异性和准确性,评价免疫组化染色诊断儿童Hp感染的价值。结果免疫组化染色诊断Hp感染的敏感性、特异性及准确性,与CIM比较差异均无统计学意义,与RUT比较其敏感性、特异性及准确性,差异也均无统计学意义。结论组织学免疫组化染色准确、可靠、易观察,是病理学诊断儿童Hp现症感染有效适宜的检测方法。
Objective To evaluate the diagnostic value of histological immunohistochemical staining for Helicobacter pylori (Hp) in children. Methods A total of 102 children with upper gastrointestinal symptoms in our pediatric department underwent gastroscopy and biopsy of antral mucosa. The rapid urease (RUT) test and immunohistochemical staining were used to detect Hp. Before gastroscopy Hp was detected by13C-urea breath test (13C-UBT) and CIM with serology. The sensitivity, specificity and accuracy of 13C-UBT were compared with those of the other two methods to evaluate the value of immunohistochemical staining in the diagnosis of Hp infection in children. Results The sensitivity, specificity and accuracy of immunohistochemical staining for Hp infection were not statistically different from those of CIM. The sensitivity, specificity, accuracy and difference of Rp were also not statistically significant. Conclusion Histological immunohistochemical staining is accurate, reliable and easy to observe. It is an effective and appropriate method for pathological diagnosis of Hp infection in children.