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目的:以四川江油栽培乌头为研究对象,探索乌头叶面微生物菌群动态变化特征及其与乌头霜霉病的相关性。方法:收集不同病害等级的乌头霜霉病叶片,摇床震荡与超声结合的方法提取乌头叶面微生物,稀释平板计数法测定乌头叶面细菌,真菌数量。结果:乌头霜霉病发病情况与环境温度相关,患病乌头叶面细菌,真菌数量相较于健康乌头都有所增加,且叶面其他真菌数量随着病害程度的逐渐加重而呈现出较有规律的逐渐增加趋势。结论:乌头霜霉病在其生长期的3月末至5月末发病迅速,且日平均气温15~20℃比较适宜乌头霜霉病病原菌的生长繁殖。乌头叶面真菌数量的变化与乌头霜霉病病害程度呈正相关。
OBJECTIVE: To investigate the dynamic changes of the microflora in the head of Aconiti kusnezoffii and its relationship with downy mildew caused by aconite in Wujiang, Sichuan Province. Methods: The leaves of aconite downy mildew with different disease grade were collected, and the combination of shaking and ultrasonic shaking was used to extract the microorganisms from the surface of aconite. The number of bacteria and fungi in the leaf surface of aconite was determined by the dilution plate counting method. Results: The incidence of downy mildew was related to the ambient temperature, and the number of fungi and fungi on the diseased camellia leaf surface was increased compared with healthy Aconitum, and the number of other fungi on the foliage was increased with the severity of the disease A more regular trend of gradual increase. CONCLUSION: Downy mildew of Aconite is very rapid in the late March to the end of May in the growing season, and the average daily temperature of 15 ~ 20 ℃ is more suitable for the growth and reproduction of the pathogens of Downy mildew. The change of the number of fungi in the head of Aconiti kusnezoffii was positively correlated with the degree of the disease.