论文部分内容阅读
目的:从大黄苷元联合尿激酶溶栓对脑缺血大鼠脑组织IgG,IV型胶原(CoLIV)和层粘蛋白(LN)变化的影响方面探讨其对脑缺血微血管基底膜损伤的保护作用。方法:大鼠随机分组,制备血栓栓塞性脑缺血动物模型,大鼠分别于缺血后3,6,9 h经导管由区域动脉溶栓;动脉给药后24 h,观察大鼠死亡率和脑组织颅内出血率,测定脑组织IgG,CoLIV和LN。结果:脑缺血9 h溶栓可引起大鼠死亡率和脑出血率增高,联合用药可使其降低。模型6 h和9 h组大鼠脑组织IgG水平增高、CoLIV和LN表达降低;各用药9 h组较模型组IgG降低、CoLIV和LN表达增强;联合用药9 h组分别较两单一用药组IgG降低、CoLIV和LN表达增强。结论:脑缺血延迟溶栓可造成脑微血管基底膜损伤,引起颅内出血率和死亡率增高;大黄苷元联合溶栓对微血管基底膜损伤具有保护作用,可降低溶栓后的颅内出血率和死亡率。
Objective: To explore the protective effect of rhubarb aglycone combined with urokinase thrombolysis on changes of IgG, type IV collagen (CoLIV) and laminin (LN) in brain tissue of cerebral ischemia rats. effect. METHODS: Rats were randomly divided into groups to prepare an animal model of thromboembolic cerebral ischemia. Rats were treated with regional arterial thrombolysis at 3, 6, and 9 h after ischemia, and 24 h after arterial dosing. Rat mortality was observed. And brain tissue intracranial hemorrhage, determination of brain tissue IgG, CoLIV and LN. RESULTS: Thrombolysis after 9 h of cerebral ischemia could increase the mortality and cerebral hemorrhage rate in rats, which could be reduced by combination therapy. The levels of IgG in brain and the expression of CoLIV and LN were decreased in 6 h and 9 h groups. The levels of CoLIV and LN in the 9 h group were lower than those in the model group, and the expression of CoLIV and LN was higher in the 9 h group than in the two groups. Reduced, increased expression of CoLIV and LN. Conclusion: Delayed thrombolysis of cerebral ischemia can cause damage to the cerebral microvascular basement membrane, which causes increased intracranial hemorrhage and mortality. Rhubarb aglycone combined with thrombolysis has a protective effect on microvascular basement membrane damage, and can reduce the intracranial hemorrhage rate after thrombolysis. mortality rate.