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目的 :建立三元络合物比色法测定复方苦参洗剂中小檗碱的含量 ,为制剂质量控制提供依据。方法 :小檗碱在 pH6 .7的溶液中 ,与溴酚蓝、奎宁形成三元络合物比色 ,经氯仿提取 ,在 6 10nm波长处测定吸收度。结果 :回归方程为A =0 .182C - 0 .0 0 4 (r =0 .9999) ,小檗碱在 0 .8～ 4mg·L-1浓度范围内线性关系良好 (r =0 .9999) ,平均回收率为 98.6 5 % ,RSD为 1.12 %。结论 :本方法准确 ,灵敏度好 ,可用于复方苦参洗剂中小檗碱的含量测定。
Objective : To establish a ternary complex colorimetric method for the determination of berberine in compound Kushen lotion, and to provide basis for quality control of preparations. METHODS: Berberine was colorimetrically formed with bromophenol blue and quinine in a solution of pH 6.7, extracted by chloroform, and the absorbance was measured at a wavelength of 610 nm. Results: The regression equation was A = 0.182C - 0 .0 0 4 (r = .9999), and the berberine had good linearity in the range of 0.8 - 4 mg.L-1 (r = .9999). The average recovery rate was 98.65% and the RSD was 1.12%. Conclusion : This method is accurate and has good sensitivity. It can be used to determine the content of berberine in compound Kushen lotion.