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目的:建立生精胶囊中主要有效成分淫羊藿苷的含量测定方法。方法:采用高效液相色谱法测定淫羊藿苷的含量,色谱柱为phenomenex-Genmini-C18(4.6mm×150mm),流动相为甲醇-水(52∶48),流速为1.0mL/min,检测波长为270nm。结果:淫羊藿苷在0.25~4μg范围内线性关系良好,回收率为100.4%,RSD为2.5%,生精胶囊中的淫羊藿苷的含量为0.37μg/g。结论:高效液相色谱法简单,结果准确,重复性好,适用于生精胶囊中的淫羊藿苷的含量测定,可以为该制剂的质量控制提供科学依据。
Objective: To establish a method for determining the content of icariin, the main effective ingredient in Shengjing capsules. Methods: The content of icariin was determined by high performance liquid chromatography. The chromatographic column was phenomenex-Genmini-C18(4.6mm×150mm), the mobile phase was methanol-water(52∶48), the flow rate was 1.0mL/min. The detection wavelength is 270 nm. RESULTS: The icariin had good linearity in the range of 0.25 to 4 μg, with a recovery of 100.4% and an RSD of 2.5%. The content of icariin in the spermatogenic capsule was 0.37 μg/g. Conclusion: High performance liquid chromatography is simple, accurate and reproducible. It is suitable for the determination of icariin in Shengjing capsules and can provide scientific basis for the quality control of the preparation.