Through a modified agar well diffusion assay,antagonism of a novel chitinase-producing strain C3 against the phy- topathogenic fungi including Phoma wasabiae Yokogi,Cochlibolus Heterostrophus,Exserohilum Turcicum,Curuvularia Lunata (Walk)Boed,Thantephorus cucumris,Fusarium graminearum was tested.The data showed that the crude extracts of strain C3 had stable antifungal activity in the range of pH 5.0 to pH 8.0.The active components were heat labile and sensitive to proteinase K.A series of experiments supported that the compound responsible for inhibitory aetivity appeared to be chitinase.The 16s rDNA analysis indicated that C3 was subject to genus Burkholderia.Phenotypic characterization of C3 was also consisted with the result of molecular identification. Through a modified agar well diffusion assay, antagonism of a novel chitinase-producing strain C3 against the phy- topathogenic fungi including Phoma wasabiae Yokogi, Cochlibolus Heterostrophus, Exserohilum Turcicum, Curuvularia Lunata (Walk) Boed, Thantephorus cucumris, Fusarium graminearum was tested.The Data showed that the crude extracts of strain C3 had stable antifungal activity in the range of pH 5.0 to pH 8.0. The active components were heat labile and sensitive to proteinase KA series of experiments supported that the compound responsible for inhibitory aetivity appeared to be chitinase. The 16s rDNA analysis indicated that C3 was subject to genus Burkholderia. Phenotypic characterization of C3 was also consisted with the result of molecular identification.