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作者用~(125)I标记纯化的LSP,建立了葡萄球菌A蛋白放射免疫沉淀法(SPA-RIA)检测抗LSP抗体,经182例各型肝炎、肝硬化,40例其它疾患,20例健康人的观察,证明本法灵敏,特异性较好。同时建立了酶联免疫吸附试验(ELISA)检测抗LSP抗体,并将抗LSP抗体进一步分为IgG、IgM及IgA型,经182例各型肝炎,37例其它疾患及50例正常人观察,证明比方法稳定可靠。用SPA-RIA及ELISA检测结果,发现急性肝炎患者的抗-LSP阳性率分别为66.2%(43/65例)及80.4%(37/46例),两种方法
The purified LSP was labeled with ~ (125) I, and the staphylococcal protein A radioimmunoprecipitation (SPA-RIA) was used to detect anti-LSP antibody. After 182 cases of various types of hepatitis, cirrhosis, 40 other diseases and 20 cases of healthy Human observation, that the law sensitive, better specificity. At the same time, the enzyme-linked immunosorbent assay (ELISA) was used to detect the anti-LSP antibodies and the anti-LSP antibodies were further divided into IgG, IgM and IgA types. The observation of 182 cases of various types of hepatitis, 37 cases of other diseases and 50 cases of normal people showed Than the method is stable and reliable. The results of SPA-RIA and ELISA showed that the positive rates of anti-LSP in patients with acute hepatitis were 66.2% (43/65 cases) and 80.4% (37/46 cases), respectively. Two methods