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本文以体外培养的人食管癌(ECa109株)单层细胞及球体为模型,从细胞动力学角度考虑,比较分属周期非特异性药物和周期特异性药物的卡氮芥(BCNU)与氟尿嘧啶(5—FU)不同合并方案对两模型细胞的杀伤作用,以选出最佳给药方案,为临床合理的合并用药提供一定依据。 材料和方法 人食管癌细胞由中国医学科学院肿瘤研究所提供。细胞常规培养。 球体的制作:取2g链霉素小瓶,底上铺2％琼脂D—Hanks液1.5ml,高压灭菌,冷却后加盖
In this paper, we use in vitro cultured human esophageal cancer (ECa109 strain) monolayer cells and spheres as a model. From the perspective of cell dynamics, we compare the cardiomycosis mustard (BCNU) and fluorouracil (5) which belong to the cycle-specific non-specific drugs and cycle-specific drugs. —FU) The effect of different combinations on the killing of two model cells to select the optimal dosing regimen to provide a basis for clinical rational drug combination. Materials and Methods Human esophageal cancer cells were provided by Cancer Institute of Chinese Academy of Medical Sciences. The cells are routinely cultured. Preparation of spheres: Take 2g of streptomycin vials, spread 2% agar D-Hanks liquid 1.5ml on the bottom, autoclave, cap after cooling