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作者建立改良的测定人血清中普罗帕酮反相ＨＰＬＣ法，固定相为ＬｉｃｈｒｏｓｏｒｂＲＰ－１８，流动相为甲醇：醋酸钠缓冲液：二乙胺（７４．５：２５：０．５ｖ／ｖ），流速为１ｍｌ／ｍｉｎ，以安定为内标，紫外检测波长２５０ｎｍ。普罗帕酮相对回收率９６．２５％～１０４．００％。日内和日间变异系数３．６％～７．８％，最低检测限为５ｎｇ，血浆中最低检测浓度４０ｎｇ／ｍｌ，在５０～３２００ｎｇ／ｍｌ浓度范围内线性良好，相关系数ｒ＝０．９９９８。用本方法测定１６名老年心律失常患者口服不同剂量普罗帕酮血浓，对老年患者服用普罗帕酮剂量─血浓─临床症状之间的关系作了观察。
The authors established an improved RP-HPLC assay of propafenone in human serum with a stationary phase of Lichrosorb RP-18 and a mobile phase of methanol: sodium acetate buffer: diethylamine (74.5: 25: 0.5 v / v) Flow rate of 1ml / min, to stability as an internal standard, UV detection wavelength of 250nm. The relative recovery of propafenone was 96.25% ~ 104.00%. The intra-and inter-day coefficient of variation was 3.6% -7.8%, the lowest detection limit was 5 ng and the lowest detection concentration in plasma was 40 ng / ml. The linearity was good in the range of 50 ~ 3200 ng / ml with a correlation coefficient of 0.9998 . Using this method, 16 elderly patients with arrhythmia were randomized to oral propafenone at different doses of propofol, and the relationship between propafenone dosage-blood concentration-clinical symptoms in elderly patients was observed.