[目的]研究4种常用抗肿瘤药物(长春新碱、平阳霉素、顺铂、氟尿嘧啶)对鼻咽癌细胞的增殖抑制作用。[方法]采用MTT法检测不同浓度长春新碱、平阳霉素、顺铂、5-Fu对鼻咽癌HNE-1细胞株增殖抑制率。[结果]长春新碱对细胞抑制率<20%的浓度为<50μg/ml,平阳霉素对细胞抑制率<20%的浓度为<40μg/ml,顺铂、5-Fu对细胞抑制率与浓度呈正相关。[结论]4种抗肿瘤药物对鼻咽癌HNE-1细胞株均存在不同程度的增殖抑制作用。 [Objective] The research aimed to study the inhibitory effects of four commonly used anticancer drugs (vincristine, bleomycin, cisplatin and fluorouracil) on the proliferation of nasopharyngeal carcinoma cells. [Method] The inhibitory rates of vincristine, pingyangmycin, cisplatin and 5-Fu with different concentrations on the proliferation of nasopharyngeal carcinoma HNE-1 cell line were detected by MTT assay. [Result] The concentration of vincristine to the cells with the rate of <20% was <50μg / ml, that of the bleomycin to the cells with the rate of <20% was <40μg / ml. Concentration was positively correlated. [Conclusion] The four anti-tumor drugs have different degrees of proliferation inhibition on NPC HNE-1 cell line.