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目的观察竹叶黄酮对乙醇诱导小鼠急性胃黏膜损伤的保护作用。方法取40只小鼠随机均分为对照组、模型组、维生素E(50 mg/kg)组以及竹叶黄酮25、50 mg/kg组。各组连续ig给药7 d,1次/d。末次给药1 h后,各组按10 mg/kg ig给予无水乙醇,制作胃黏膜损伤模型。建模4 h后,观察胃黏膜损伤面积,计算胃黏膜损伤指数和损伤抑制率。测定小鼠胃黏膜组织中一氧化氮(NO)、前列腺素(PEG2)含量以及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果与模型组比较,竹叶黄酮25 mg/kg组小鼠胃黏膜损伤面积减少,50 mg/kg组小鼠损伤程度最小。与模型组比较,竹叶黄酮25、50 mg/kg组小鼠胃黏膜损伤指数均明显降低(P<0.01);胃黏膜组织NO、PEG_2含量明显升高(P<0.01);血清SOD、MDA水平明显降低(P<0.01),SOD/MDA比值明显升高(P<0.01)。结论竹叶黄酮能拮抗乙醇诱导的胃黏膜损伤,其机制可能与其抗氧化、抑制氧自由基产生并升高胃黏膜NO、PEG_2含量有关。
Objective To observe the protective effect of bamboo leaf flavonoids on alcohol induced acute gastric mucosal injury in mice. Methods 40 mice were randomly divided into control group, model group, vitamin E (50 mg / kg) group and bamboo leaf flavone 25,50 mg / kg group. Each group of continuous ig administration 7 d, 1 time / d. 1 h after the last administration, each group given 10 mg / kg ig given ethanol, making a model of gastric mucosal injury. After modeling for 4 h, the area of gastric mucosal injury was observed, and the gastric mucosal injury index and injury inhibition rate were calculated. The content of nitric oxide (NO), prostaglandin (PEG2) and the levels of serum superoxide dismutase (SOD) and malondialdehyde (MDA) in gastric mucosa of mice were measured. Results Compared with the model group, the gastric mucosal lesion area of mice treated with bamboo leaves flavonoids at 25 mg / kg was reduced, and the damage at 50 mg / kg was the smallest. Compared with the model group, the gastric mucosal lesion index of bamboo leaves flavonoids 25 and 50 mg / kg groups were significantly decreased (P <0.01), while the content of NO and PEG 2 in gastric mucosa significantly increased (P <0.01) (P <0.01), and the ratio of SOD / MDA increased significantly (P <0.01). Conclusion Bamboo leaves flavonoids can antagonize ethanol-induced gastric mucosal injury, its mechanism may be related to its anti-oxidation, inhibition of oxygen free radicals and increased gastric mucosal NO, PEG 2 content.