论文部分内容阅读
目的了解青岛地区病毒性腹泻患者中诺如病毒的感染情况,明确该地区诺如病毒的主要基因分型和分子进化特点。方法收集青岛市青岛医学院附属医院散发疑似病毒性腹泻粪便标本94例,应用逆转录-聚合酶链反应对诺如病毒进行检测。结果检测到诺如病毒2株,对其进一步进行系统进化分析,表明这2株诺如病毒属于基因型GI-8和G1-9。结论青岛地区存在诺如病毒感染,并已查明其基因型。
Objective To understand the infection of norovirus in patients with viral diarrhea in Qingdao and to identify the main genotyping and molecular evolution characteristics of norovirus in this area. Methods Ninety - four cases of suspected viral diarrhea were collected from the Affiliated Hospital of Qingdao Medical College of Qingdao City. Norovirus was detected by reverse transcription - polymerase chain reaction. Results Two Norovirus strains were detected and further phylogenetic analysis was performed, indicating that the two Norovirus strains belonged to the genotypes GI-8 and G1-9. Conclusion Norovirus infection exists in Qingdao and genotypes have been identified.